› 6vcs

X-ray diffraction
1.7Å resolution

SRA domain of UHRF1 in complex with DNA

Released:
DOI: 10.2210/pdb6vcs/pdb
PDB entry 6vcs coloured by chain, front view.
PDB entry 6vcs coloured by chain, side view.
PDB entry 6vcs coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Dong C, Tempel W, Bountra C, Edwards AM, Arrowsmith CH, Min J, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120753 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
E3 ubiquitin-protein ligase UHRF1 Chains: A, B, E
Molecule details ›
Chains: A, B, E
Length: 211 amino acids
Theoretical weight: 23.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96T88 (Residues: 414-617; Coverage: 26%)
Gene names: ICBP90, NP95, RNF106, UHRF1
Sequence domains: SAD/SRA domain
UNK-UNK-UNK-UNK Chain: G
Molecule details ›
Chain: G
Length: 6 amino acids
Theoretical weight: 529 Da
Source organism: Homo sapiens
Expression system: Escherichia coli
DNA (5'-D(*GP*CP*CP*TP*GP*TP*AP*CP*AP*GP*GP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 nucleotides
Theoretical weight: 3.66 KDa

Ligands and Environments

1 bound ligand:
Ligand UNX 66 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: P212121
Unit cell:
a: 51.995Å b: 113.809Å c: 133.946Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.237 0.258
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

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