› 6vg2

X-ray diffraction
3.9Å resolution

Crystal structure of the DNA binding domain of human transcription factor FLI1 in complex with 16-mer DNA CAGAGGATGTGGCTTC

Released:
DOI: 10.2210/pdb6vg2/pdb
PDB entry 6vg2 coloured by chain, front view.
PDB entry 6vg2 coloured by chain, side view.
PDB entry 6vg2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric interference in oncogenic FLI1 and ERG transactions by mithramycins.
Hou C, Mandal A, Rohr J, Tsodikov OV
Structure 29 404-412.e4 (2021)
PMID: 33275876

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120770 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Friend leukemia integration 1 transcription factor Chains: A, D
Molecule details ›
Chains: A, D
Length: 104 amino acids
Theoretical weight: 12.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01543 (Residues: 276-375; Coverage: 22%)
Gene name: FLI1
Sequence domains: Ets-domain
DNA (5'-D(*GP*AP*CP*CP*GP*GP*AP*AP*GP*TP*GP*GP*CP*TP*TP*C)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 16 nucleotides
Theoretical weight: 4.95 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*AP*AP*GP*CP*CP*AP*CP*TP*TP*CP*CP*GP*GP*TP*C)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 16 nucleotides
Theoretical weight: 4.84 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I212121
Unit cell:
a: 79.422Å b: 90.732Å c: 165.204Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.247 0.294
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Current insights into the role of Fli-1 in hematopoiesis and malignant transformation.
Ben-David et al. (2022)
1 more

2 mentions without citation

Allosteric interference in oncogenic FLI1 and ERG transactions by mithramycins.
Hou et al. (2021)
1 more