› 6vg8

X-ray diffraction
4.31Å resolution

Crystal structure of the DNA binding domains of human FLI1 and Runx2 in complex with 16-mer DNA CAGAGGATGTGGCTTC

Released:
DOI: 10.2210/pdb6vg8/pdb
PDB entry 6vg8 coloured by chain, front view.
PDB entry 6vg8 coloured by chain, side view.
PDB entry 6vg8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric interference in oncogenic FLI1 and ERG transactions by mithramycins.
Hou C, Mandal A, Rohr J, Tsodikov OV
Structure 29 404-412.e4 (2021)
PMID: 33275876

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120772 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Friend leukemia integration 1 transcription factor Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 12.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01543 (Residues: 276-375; Coverage: 22%)
Gene name: FLI1
Sequence domains: Ets-domain
Runt-related transcription factor 2 Chain: D
Molecule details ›
Chain: D
Length: 123 amino acids
Theoretical weight: 13.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13950 (Residues: 111-233; Coverage: 24%)
Gene names: AML3, CBFA1, OSF2, PEBP2A, RUNX2
Sequence domains: Runt domain
DNA (5'-D(P*CP*AP*GP*AP*GP*GP*AP*TP*GP*TP*GP*GP*CP*TP*TP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.95 KDa
DNA (5'-D(P*GP*AP*AP*GP*CP*CP*AP*CP*AP*TP*CP*CP*TP*CP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.84 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6222
Unit cell:
a: 104.427Å b: 104.427Å c: 326.382Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.274 0.273 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Current insights into the role of Fli-1 in hematopoiesis and malignant transformation.
Ben-David et al. (2022)
1 more

1 mention without citation

Allosteric interference in oncogenic FLI1 and ERG transactions by mithramycins.
Hou et al. (2021)