› 6vi4

X-ray diffraction
3.3Å resolution

Nanobody-Enabled Monitoring of Kappa Opioid Receptor States

Released:
DOI: 10.2210/pdb6vi4/pdb
PDB entry 6vi4 coloured by chain, front view.
PDB entry 6vi4 coloured by chain, side view.
PDB entry 6vi4 coloured by chain, top view.
Source organisms:
Primary publication:
Nanobody-enabled monitoring of kappa opioid receptor states.
Che T, English J, Krumm BE, Kim K, Pardon E, Olsen RHJ, Wang S, Zhang S, Diberto JF, Sciaky N, Carroll FI, Steyaert J, Wacker D, Roth BL
Nat Commun 11 1145 (2020)
PMID: 32123179

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231805 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kappa-type opioid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 34.82 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41145 (Residues: 54-358; Coverage: 80%)
Gene names: OPRK, OPRK1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Nanobody 6 Chains: C, D
Molecule details ›
Chains: C, D
Length: 133 amino acids
Theoretical weight: 14.73 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand JDC 2 x JDC
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Unit cell:
a: 102.394Å b: 108.191Å c: 155.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.242 0.271
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

21 review citations

Exploring cellular biochemistry with nanobodies.
Cheloha et al. (2020)
20 more

11 mentions without citation

Allosteric Modulation of GPCRs of Class A by Cholesterol.
Jakubík et al. (2021)
10 more