› 6vjf

X-ray diffraction
2.47Å resolution

The P-Loop K to A mutation of C. therm Vps1 GTPase-BSE

Released:
DOI: 10.2210/pdb6vjf/pdb
PDB entry 6vjf coloured by chain, front view.
PDB entry 6vjf coloured by chain, side view.
PDB entry 6vjf coloured by chain, top view.
Source organism: Thermochaetoides thermophila DSM 1495
Primary publication:
Structural and functional characterization of the dominant negative P-loop lysine mutation in the dynamin superfamily protein Vps1.
Tornabene BA, Varlakhanova NV, Hosford CJ, Chappie JS, Ford MGJ
Protein Sci 29 1416-1428 (2020)
PMID: 31981262

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124102 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative sorting protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 391 amino acids
Theoretical weight: 42.94 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: G0SFF0 (Residues: 1-351, 364-698; Coverage: 55%)
Gene name: CTHT_0061810
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GCP 4 x GCP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 81.873Å b: 119.032Å c: 84.576Å
α: 90° β: 100.95° γ: 90°
R-values:
R R work R free
0.191 0.188 0.238
Expression system: Escherichia coli BL21

Quick links

Citations

4 citation in other articles

A PX-BAR protein Mvp1/SNX8 and a dynamin-like GTPase Vps1 drive endosomal recycling.
Suzuki et al. (2021)
3 more

2 mentions without citation

Structural and functional characterization of the dominant negative P-loop lysine mutation in the dynamin superfamily protein Vps1.
Tornabene et al. (2020)
1 more