› 6vks

Electron Microscopy
3.02Å resolution

Cryo-electron microscopy structures of a gonococcal multidrug efflux pump illuminate a mechanism of drug recognition with ampicillin

Released:
DOI: 10.2210/pdb6vks/pdb
PDB entry 6vks coloured by chain, front view.
PDB entry 6vks coloured by chain, side view.
PDB entry 6vks coloured by chain, top view.
Source organism: Neisseria gonorrhoeae
Primary publication:
Cryo-EM Structures of a Gonococcal Multidrug Efflux Pump Illuminate a Mechanism of Drug Recognition and Resistance.
Lyu M, Moseng MA, Reimche JL, Holley CL, Dhulipala V, Su CC, Shafer WM, Yu EW
mBio 11 (2020)
PMID: 32457251
Related structures: EMD-21228

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177066 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Efflux pump membrane transporter Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1049 amino acids
Theoretical weight: 112.04 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5F725 (Residues: 1-1049; Coverage: 98%)
Gene name: NGO_1364
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

2 bound ligands:
Ligand PTY 17 x PTY
Ligand AIX 1 x AIX
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.02Å
Relevant EMDB volumes: EMD-21228
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
5 more

7 mentions without citation

Detergent-free systems for structural studies of membrane proteins.
Guo Y. (2021)
6 more