› 6vkt

Electron Microscopy
2.72Å resolution

Cryo-electron microscopy structures of a gonococcal multidrug efflux pump illuminate a mechanism of erythromycin drug recognition

Released:
DOI: 10.2210/pdb6vkt/pdb
PDB entry 6vkt coloured by chain, front view.
PDB entry 6vkt coloured by chain, side view.
PDB entry 6vkt coloured by chain, top view.
Source organism: Neisseria gonorrhoeae
Primary publication:
Cryo-EM Structures of a Gonococcal Multidrug Efflux Pump Illuminate a Mechanism of Drug Recognition and Resistance.
Lyu M, Moseng MA, Reimche JL, Holley CL, Dhulipala V, Su CC, Shafer WM, Yu EW
mBio 11 (2020)
PMID: 32457251
Related structures: EMD-21229

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177066 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Efflux pump membrane transporter Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1046 amino acids
Theoretical weight: 111.64 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5F725 (Residues: 1-1046; Coverage: 98%)
Gene name: NGO_1364
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

2 bound ligands:
Ligand PTY 23 x PTY
Ligand ERY 1 x ERY
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.72Å
Relevant EMDB volumes: EMD-21229
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
3 more

10 mentions without citation

Ever-Adapting RND Efflux Pumps in Gram-Negative Multidrug-Resistant Pathogens: A Race against Time.
Zwama et al. (2021)
9 more