› 6vmc

X-ray diffraction
2.85Å resolution

T4H2 T cell receptor bound to HLA-A2 presenting gp100T2L peptide (ILDQVPFSV)

Released:
DOI: 10.2210/pdb6vmc/pdb
PDB entry 6vmc coloured by chain, front view.
PDB entry 6vmc coloured by chain, side view.
PDB entry 6vmc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structurally silent peptide anchor modifications allosterically modulate T cell recognition in a receptor-dependent manner
Smith AR, Alonso JA, Ayres CM, Singh NK, Hellman LM, Baker BM
Proc. Natl. Acad. Sci. U.S.A. 118 e2018125118- (2021)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-231549 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
T4H2 T cell receptor alpha chain Chain: D
Molecule details ›
Chain: D
Length: 206 amino acids
Theoretical weight: 22.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
T4H2 T cell receptor beta chain Chain: E
Molecule details ›
Chain: E
Length: 241 amino acids
Theoretical weight: 27.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A140T913 (Residues: 25-299; Coverage: 79%)
Gene name: HLA-A
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
M-alpha Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.02 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P40967 (Residues: 209-217; Coverage: 1%)
Gene names: D12S53E, PMEL, PMEL17, SILV

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P64
Unit cell:
a: 129.532Å b: 129.532Å c: 115.656Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.197 0.238
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli
  • Not provided

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