› 6vrm

X-ray diffraction
2.61Å resolution

T cell receptor-p53-HLA-A2 complex

Released:
DOI: 10.2210/pdb6vrm/pdb
PDB entry 6vrm coloured by chain, front view.
PDB entry 6vrm coloured by chain, side view.
PDB entry 6vrm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for oligoclonal T cell recognition of a shared p53 cancer neoantigen.
Wu D, Gallagher DT, Gowthaman R, Pierce BG, Mariuzza RA
Nat Commun 11 2908 (2020)
PMID: 32518267

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-231543 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 33.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A140T913 (Residues: 25-299; Coverage: 79%)
Gene name: HLA-A
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
T-cell receptor 12-6, alfa chain Chain: D
Molecule details ›
Chain: D
Length: 205 amino acids
Theoretical weight: 22.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
TCR 12-6, beta chain Chain: E
Molecule details ›
Chain: E
Length: 246 amino acids
Theoretical weight: 27.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Cellular tumor antigen p53 Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 1.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 168-176; Coverage: 2%)
Gene names: P53, TP53

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 89.405Å b: 120.608Å c: 119.693Å
α: 90° β: 108.38° γ: 90°
R-values:
R R work R free
0.195 0.19 0.275
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Targeting public neoantigens for cancer immunotherapy.
Pearlman et al. (2021)
7 more

5 mentions without citation

TCR Recognition of Peptide-MHC-I: Rule Makers and Breakers.
Szeto et al. (2020)
4 more