› 6vu0

X-ray diffraction
3.5Å resolution

CRYSTAL STRUCTURE OF THE C-TERMINAL DOMAIN OF ENZYME I OF THE BACTERIAL PHOSPHOTRANSFERASE SYSTEM FROM THE ESCHERICHIA COLI ENZYME

Released:
DOI: 10.2210/pdb6vu0/pdb
Model geometry
Data not analysed
Fit model/data
Data not analysed
PDB entry 6vu0 coloured by chain, front view.
PDB entry 6vu0 coloured by chain, side view.
PDB entry 6vu0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Hybrid Thermophilic/Mesophilic Enzymes Reveal a Role for Conformational Disorder in Regulation of Bacterial Enzyme I.
Dotas RR, Nguyen TT, Stewart CE, Ghirlando R, Potoyan DA, Venditti V
J Mol Biol 432 4481-4498 (2020)
PMID: 32504625

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate-protein phosphotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 316 amino acids
Theoretical weight: 35.35 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08839 (Residues: 261-575; Coverage: 55%)
Gene names: JW2409, b2416, ptsI
Sequence domains: PEP-utilising enzyme, PEP-binding domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P4122
Unit cell:
a: 136.46Å b: 136.46Å c: 183.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.26
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Solution NMR methods for structural and thermodynamic investigation of nanoparticle adsorption equilibria.
An et al. (2022)
1 more

2 mentions without citation

Hybrid Thermophilic/Mesophilic Enzymes Reveal a Role for Conformational Disorder in Regulation of Bacterial Enzyme I.
Dotas et al. (2020)
1 more