› 6vwk

Electron Microscopy
3.3Å resolution

E. coli ATP Synthase ADP Sub-state 3a Fo Focussed

Released:
DOI: 10.2210/pdb6vwk/pdb
PDB entry 6vwk coloured by chain, front view.
PDB entry 6vwk coloured by chain, side view.
PDB entry 6vwk coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Cryo-EM structures provide insight into how E. coli F1Fo ATP synthase accommodates symmetry mismatch.
Sobti M, Walshe JL, Wu D, Ishmukhametov R, Zeng YC, Robinson CV, Berry RM, Stewart AG
Nat Commun 11 2615 (2020)
PMID: 32457314
Related structures: EMD-21419

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142031 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP synthase subunit c Chains: I, J, L, M, N, O, P, Q, R, S
Molecule details ›
Chains: I, J, L, M, N, O, P, Q, R, S
Length: 79 amino acids
Theoretical weight: 8.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P68699 (Residues: 1-79; Coverage: 100%)
Gene names: JW3715, atpE, b3737, papH, uncE
Sequence domains: ATP synthase subunit C
ATP synthase subunit b Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 156 amino acids
Theoretical weight: 17.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABA0 (Residues: 1-156; Coverage: 100%)
Gene names: JW3714, atpF, b3736, papF, uncF
Sequence domains: ATP synthase B/B' CF(0)
ATP synthase subunit a Chain: a
Molecule details ›
Chain: a
Length: 271 amino acids
Theoretical weight: 30.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AB98 (Residues: 1-271; Coverage: 100%)
Gene names: JW3716, atpB, b3738, papD, uncB
Sequence domains: ATP synthase A chain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-21419
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
11 more

3 mentions without citation

Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases.
Valdivieso González et al. (2023)
2 more