› 6vyv

Electron Microscopy
6.33Å resolution

Human mAbs broadly protect against infection of arthritiogenic alphaviruses by recognizing conserved elements of the MXR8 receptor binding domain

Released:
DOI: 10.2210/pdb6vyv/pdb
PDB entry 6vyv coloured by chain, front view.
PDB entry 6vyv coloured by chain, side view.
PDB entry 6vyv coloured by chain, top view.
Source organisms:
Primary publication:
Human mAbs Broadly Protect against Arthritogenic Alphaviruses by Recognizing Conserved Elements of the Mxra8 Receptor-Binding Site.
Powell LA, Miller A, Fox JM, Kose N, Klose T, Kim AS, Bombardi R, Tennekoon RN, Dharshan de Silva A, Carnahan RH, Diamond MS, Rossmann MG, Kuhn RJ, Crowe JE
Cell Host Microbe 28 699-711.e7 (2020)
PMID: 32783883
Related structures: EMD-21473

Function and Biology Details

Reaction catalysed: 
Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 960-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209365 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Spike glycoprotein E1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 393 amino acids
Theoretical weight: 42.72 KDa
Source organism: Ross river virus (STRAIN T48)
Expression system: Mesocricetus auratus
UniProt:
  • Canonical: P08491 (Residues: 817-1209; Coverage: 31%)
Sequence domains: Alphavirus E1 glycoprotein
Spike glycoprotein E2 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 341 amino acids
Theoretical weight: 38.24 KDa
Source organism: Ross river virus (STRAIN T48)
Expression system: Mesocricetus auratus
UniProt:
  • Canonical: P08491 (Residues: 335-675; Coverage: 27%)
Sequence domains: Alphavirus E2 glycoprotein
Fab CHK-265 heavy chain Chains: I, J, K, L
Fab CHK-265 light chain Chains: M, N, O, P

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 6.33Å
Relevant EMDB volumes: EMD-21473
Expression systems:
  • Mesocricetus auratus
  • Homo sapiens

Quick links

Citations

8 review citations

Requirement of Fc-Fc Gamma Receptor Interaction for Antibody-Based Protection against Emerging Virus Infections.
Keeler et al. (2021)
7 more

2 mentions without citation

Immunoinformatics approach to understand molecular interaction between multi-epitopic regions of SARS-CoV-2 spike-protein with TLR4/MD-2 complex.
Bhattacharya et al. (2020)
1 more