› 6vzr

X-ray diffraction
2.6Å resolution

Engineered TTLL6 bound to the initiation analog

Released:
DOI: 10.2210/pdb6vzr/pdb
PDB entry 6vzr coloured by chain, front view.
PDB entry 6vzr coloured by chain, side view.
PDB entry 6vzr coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for polyglutamate chain initiation and elongation by TTLL family enzymes.
Mahalingan KK, Keith Keenan E, Strickland M, Li Y, Liu Y, Ball HL, Tanner ME, Tjandra N, Roll-Mecak A
Nat Struct Mol Biol 27 802-813 (2020)
PMID: 32747782

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-218859 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin polyglutamylase TTLL6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 453 amino acids
Theoretical weight: 53.35 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: A4Q9E8 (Residues: 51-503; Coverage: 55%)
Gene name: Ttll6
Sequence domains: Tubulin-tyrosine ligase family
TTLL6 unregistered chain Chain: F
Molecule details ›
Chain: F
Length: 12 amino acids
Theoretical weight: 1.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
TTLL6 unregistered chain Chain: G
Molecule details ›
Chain: G
Length: 11 amino acids
Theoretical weight: 954 Da
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand 2TI 4 x 2TI
Ligand ADP 4 x ADP
Ligand GOL 26 x GOL
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 75.099Å b: 108.813Å c: 171.618Å
α: 90° β: 90.03° γ: 90°
R-values:
R R work R free
0.231 0.23 0.249
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Evolution of glutamatergic signaling and synapses.
Moroz et al. (2021)
5 more