› 6vzv

X-ray diffraction
2.33Å resolution

TTLL6 bound to gamma-elongation analog

Released:
DOI: 10.2210/pdb6vzv/pdb
PDB entry 6vzv coloured by chain, front view.
PDB entry 6vzv coloured by chain, side view.
PDB entry 6vzv coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for polyglutamate chain initiation and elongation by TTLL family enzymes.
Mahalingan KK, Keith Keenan E, Strickland M, Li Y, Liu Y, Ball HL, Tanner ME, Tjandra N, Roll-Mecak A
Nat Struct Mol Biol 27 802-813 (2020)
PMID: 32747782

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-218861 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin polyglutamylase TTLL6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 453 amino acids
Theoretical weight: 53.37 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: A4Q9E8 (Residues: 51-503; Coverage: 55%)
Gene name: Ttll6
Sequence domains: Tubulin-tyrosine ligase family
TTLL6 unregistered chain Chain: I
Molecule details ›
Chain: I
Length: 15 amino acids
Theoretical weight: 1.29 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
TTLL6 unregistered chain Chain: J
Molecule details ›
Chain: J
Length: 16 amino acids
Theoretical weight: 1.38 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

5 bound ligands:
Ligand S3A 4 x S3A
Ligand ADP 4 x ADP
Ligand MG 8 x MG
Ligand GOL 32 x GOL
Ligand S37 2 x S37
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 75.072Å b: 109.716Å c: 173.032Å
α: 90° β: 90.26° γ: 90°
R-values:
R R work R free
0.183 0.182 0.211
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Evolution of glutamatergic signaling and synapses.
Moroz et al. (2021)
5 more