› 6w1a

X-ray diffraction
2.8Å resolution

Crystal structure of Streptococcus dysgalactiae SHP pheromone receptor Rgg2 bound to DNA

Released:
DOI: 10.2210/pdb6w1a/pdb
PDB entry 6w1a coloured by chain, front view.
PDB entry 6w1a coloured by chain, side view.
PDB entry 6w1a coloured by chain, top view.
Source organism: Streptococcus dysgalactiae
Primary publication:
Structure-function studies of Rgg binding to pheromones and target promoters reveal a model of transcription factor interplay.
Capodagli GC, Tylor KM, Kaelber JT, Petrou VI, Federle MJ, Neiditch MB
Proc Natl Acad Sci U S A 117 24494-24502 (2020)
PMID: 32907945

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102278 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 33.54 KDa
Source organism: Streptococcus dysgalactiae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0J9X288 (Residues: 1-284; Coverage: 100%)
Gene name: mutR
Sequence domains:
DNA (30-MER) Chain: E
Molecule details ›
Chain: E
Length: 30 nucleotides
Theoretical weight: 9.04 KDa
Source organism: Streptococcus dysgalactiae
Expression system: Not provided
DNA (30-MER) Chain: F
Molecule details ›
Chain: F
Length: 30 nucleotides
Theoretical weight: 9.4 KDa
Source organism: Streptococcus dysgalactiae
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P65
Unit cell:
a: 110.269Å b: 110.269Å c: 173.984Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.234 0.264
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Using cryo-EM to uncover mechanisms of bacterial transcriptional regulation.
Wood et al. (2021)
4 other articles

2 mentions without citation

Molecular mechanism of quorum sensing inhibition in Streptococcus by the phage protein paratox.
Rutbeek et al. (2021)
1 more