› 6w1s

Electron Microscopy
4.02Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 25-mer (preferred)
PDBe Complex ID:
PDB-CPX-106807 (preferred)
Entry contents:
25 distinct polypeptide molecules
Macromolecules (25 distinct):
Mediator of RNA polymerase II transcription subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 32.53 KDa
Source organism: Mus musculus
Mediator of RNA polymerase II transcription subunit 4 Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.65 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CQA5 (Residues: 30-129; Coverage: 37%)
Gene names: Med4, Vdrip
Sequence domains: Vitamin-D-receptor interacting Mediator subunit 4
Mediator of RNA polymerase II transcription subunit 6 Chain: C
Molecule details ›
Chain: C
Length: 246 amino acids
Theoretical weight: 28.47 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q921D4 (Residues: 1-246; Coverage: 100%)
Gene name: Med6
Sequence domains: MED6 mediator sub complex component
Mediator of RNA polymerase II transcription subunit 7 Chain: D
Molecule details ›
Chain: D
Length: 157 amino acids
Theoretical weight: 18.84 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CZB6 (Residues: 11-167; Coverage: 67%)
Gene names: Crsp9, Med7
Sequence domains: MED7 protein
Mediator of RNA polymerase II transcription subunit 8 Chain: E
Molecule details ›
Chain: E
Length: 180 amino acids
Theoretical weight: 20.16 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9D7W5 (Residues: 7-186; Coverage: 67%)
Gene names: MNCb-2386, Med8
Sequence domains: Mediator of RNA polymerase II transcription complex subunit 8
Mediator of RNA polymerase II transcription subunit 9 Chain: F
Molecule details ›
Chain: F
Length: 76 amino acids
Theoretical weight: 8.92 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q8VCS6 (Residues: 61-136; Coverage: 54%)
Gene names: Med25, Med9
Sequence domains: RNA polymerase II transcription mediator complex subunit 9
Mediator of RNA polymerase II transcription subunit 10 Chain: G
Molecule details ›
Chain: G
Length: 155 amino acids
Theoretical weight: 15.95 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CXU0 (Residues: 51-135; Coverage: 63%)
Gene names: D13Wsu50e, Med10
Sequence domains: Transcription factor subunit Med10 of Mediator complex
Mediator of RNA polymerase II transcription subunit 11 Chain: H
Molecule details ›
Chain: H
Length: 108 amino acids
Theoretical weight: 12.17 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9D8C6 (Residues: 8-115; Coverage: 92%)
Gene name: Med11
Sequence domains: Mediator complex protein
Mediator of RNA polymerase II transcription subunit 14 Chain: I
Molecule details ›
Chain: I
Length: 1548 amino acids
Theoretical weight: 168.72 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: A2ABV5 (Residues: 1-1459; Coverage: 100%)
Gene names: Crsp2, Gm641, Med14, Trap170
Sequence domains: Mediator complex subunit MED14
Mediator of RNA polymerase II transcription subunit 15 Chain: J
Molecule details ›
Chain: J
Length: 167 amino acids
Theoretical weight: 18.9 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q924H2 (Residues: 620-786; Coverage: 21%)
Gene names: Med15, Pcqap
Sequence domains: ARC105/Med15 mediator subunit C-terminal domain
Mediator of RNA polymerase II transcription subunit 16 Chain: K
Molecule details ›
Chain: K
Length: 828 amino acids
Theoretical weight: 91.89 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q6PGF3 (Residues: 1-828; Coverage: 100%)
Gene names: Med16, Thrap5
Sequence domains:
Mediator of RNA polymerase II transcription subunit 17 Chain: L
Molecule details ›
Chain: L
Length: 631 amino acids
Theoretical weight: 70.67 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q8VCD5 (Residues: 15-645; Coverage: 97%)
Gene names: Crsp6, Med17, Trap80
Sequence domains: Subunit 17 of Mediator complex
Mediator of RNA polymerase II transcription subunit 18 Chain: M
Molecule details ›
Chain: M
Length: 187 amino acids
Theoretical weight: 21.43 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CZ82 (Residues: 17-203; Coverage: 90%)
Gene name: Med18
Sequence domains: Med18 protein
Mediator of RNA polymerase II transcription subunit 19 Chain: N
Molecule details ›
Chain: N
Length: 243 amino acids
Theoretical weight: 24.68 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q8C1S0 (Residues: 1-244; Coverage: 100%)
Gene name: Med19
Sequence domains: Mediator of RNA pol II transcription subunit 19
Mediator of RNA polymerase II transcription subunit 20 Chain: O
Molecule details ›
Chain: O
Length: 198 amino acids
Theoretical weight: 21.66 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9R0X0 (Residues: 3-200; Coverage: 93%)
Gene names: Med20, Trfp
Sequence domains: TATA-binding related factor (TRF) of subunit 20 of Mediator complex
Mediator of RNA polymerase II transcription subunit 21 Chain: P
Molecule details ›
Chain: P
Length: 126 amino acids
Theoretical weight: 13.62 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CQ39 (Residues: 3-128; Coverage: 88%)
Gene names: Med21, Srb7, Surb7
Sequence domains: Subunit 21 of Mediator complex
Mediator of RNA polymerase II transcription subunit 22 Chain: Q
Molecule details ›
Chain: Q
Length: 131 amino acids
Theoretical weight: 15.28 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q62276 (Residues: 9-139; Coverage: 66%)
Gene names: Med22, Surf5
Sequence domains: Surfeit locus protein 5 subunit 22 of Mediator complex
Mediator of RNA polymerase II transcription subunit 23 Chain: R
Molecule details ›
Chain: R
Length: 1367 amino acids
Theoretical weight: 156.27 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q80YQ2 (Residues: 1-1367; Coverage: 100%)
Gene names: Crsp3, Kiaa1216, Med23, Sur2
Sequence domains: Mediator complex subunit 23
Mediator of RNA polymerase II transcription subunit 24 Chain: S
Molecule details ›
Chain: S
Length: 982 amino acids
Theoretical weight: 109.56 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q99K74 (Residues: 4-985; Coverage: 100%)
Gene names: D11Ertd307e, Med24, Thrap4, Trap100
Sequence domains: Mediator complex subunit 24 N-terminal
Mediator of RNA polymerase II transcription subunit 25 Chain: T
Molecule details ›
Chain: T
Length: 202 amino acids
Theoretical weight: 22.03 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q8VCB2 (Residues: 15-216; Coverage: 27%)
Gene name: Med25
Sequence domains: Mediator complex subunit 25 von Willebrand factor type A
Mediator of RNA polymerase II transcription subunit 27 Chain: V
Molecule details ›
Chain: V
Length: 297 amino acids
Theoretical weight: 33.74 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9DB40 (Residues: 8-304; Coverage: 96%)
Gene names: Crsp8, Med27
Sequence domains: Mediator complex subunit 27
Mediator of RNA polymerase II transcription subunit 28 Chain: W
Molecule details ›
Chain: W
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q920D3 (Residues: 32-149; Coverage: 66%)
Gene names: Fksg20, Med28
Sequence domains: Mediator complex subunit 28
Mediator of RNA polymerase II transcription subunit 29 Chain: X
Molecule details ›
Chain: X
Length: 134 amino acids
Theoretical weight: 14.97 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9DB91 (Residues: 52-185; Coverage: 67%)
Gene names: Ixl, Med29
Sequence domains: Mediator complex subunit 29
Mediator of RNA polymerase II transcription subunit 30 Chain: Y
Molecule details ›
Chain: Y
Length: 152 amino acids
Theoretical weight: 17.87 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CQI9 (Residues: 27-178; Coverage: 85%)
Gene names: Med30, Thrap6, Trap25
Sequence domains: Mediator complex subunit 30
Mediator of RNA polymerase II transcription subunit 31 Chain: Z
Molecule details ›
Chain: Z
Length: 93 amino acids
Theoretical weight: 11.55 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CXU1 (Residues: 13-105; Coverage: 71%)
Gene names: Med31, Soh1
Sequence domains: SOH1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.02Å
Relevant EMDB volumes: EMD-21514

Quick links

Citations

33 review citations

Structure and mechanism of the RNA polymerase II transcription machinery.
Schier et al. (2020)
32 more

5 mentions without citation

Genetically Engineered Mice Unveil In Vivo Roles of the Mediator Complex.
Ilchuk et al. (2023)
4 more