› 6w3z

X-ray diffraction
2.3Å resolution

Crystal Structure of Brugia malayi Deoxyhypusine synthase (DHPS)

Released:
DOI: 10.2210/pdb6w3z/pdb
PDB entry 6w3z coloured by chain, front view.
PDB entry 6w3z coloured by chain, side view.
PDB entry 6w3z coloured by chain, top view.
Source organism: Brugia malayi
Primary publication:
Structural features and development of an assay platform of the parasite target deoxyhypusine synthase of Brugia malayi and Leishmania major.
Silva SF, Klippel AH, Ramos PZ, Santiago ADS, Valentini SR, Bengtson MH, Massirer KB, Bilsland E, Couñago RM, Zanelli CF
PLoS Negl Trop Dis 14 e0008762 (2020)
PMID: 33044977

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102285 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyhypusine synthase, putative Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 367 amino acids
Theoretical weight: 41.13 KDa
Source organism: Brugia malayi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0J9XTC4 (Residues: 1-366; Coverage: 100%)
Gene names: BM_Bm14681, Bm14681, Bma-dhps-1, bma-dhps-1
Sequence domains: Deoxyhypusine synthase

Ligands and Environments

3 bound ligands:
Ligand NAD 3 x NAD
Ligand GOL 5 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 69.753Å b: 136.32Å c: 75.883Å
α: 90° β: 92.92° γ: 90°
R-values:
R R work R free
0.189 0.188 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The eukaryotic initiation factor 5A (eIF5A1), the molecule, mechanisms and recent insights into the pathophysiological roles.
Tauc et al. (2021)
1 other article

1 mention without citation

Structural features and development of an assay platform of the parasite target deoxyhypusine synthase of Brugia malayi and Leishmania major.
Silva et al. (2020)