› 6w51

X-ray diffraction
3.53Å resolution

Structure of the antibody fragment H2 in complex with HLA-A*02:01/p53R175H

Released:
DOI: 10.2210/pdb6w51/pdb
Model geometry
Fit model/data
PDB entry 6w51 coloured by chain, front view.
PDB entry 6w51 coloured by chain, side view.
PDB entry 6w51 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Targeting a neoantigen derived from a common TP53 mutation.
Hsiue EH, Wright KM, Douglass J, Hwang MS, Mog BJ, Pearlman AH, Paul S, DiNapoli SR, Konig MF, Wang Q, Schaefer A, Miller MS, Skora AD, Azurmendi PA, Murphy MB, Liu Q, Watson E, Li Y, Pardoll DM, Bettegowda C, Papadopoulos N, Kinzler KW, Vogelstein B, Gabelli SB, Zhou S
Science 371 (2021)
PMID: 33649166

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-207349 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 296 amino acids
Theoretical weight: 34.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140T913 (Residues: 25-300; Coverage: 80%)
Gene name: HLA-A
Sequence domains:
Beta-2-microglobulin Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 119 amino acids
Theoretical weight: 13.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 1-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Cellular tumor antigen p53 Chains: C, F, I, L
Immunoglobulin heavy chain H2 Chains: M, O, Q, S
Immunoglobulin light chain H2 Chains: N, P, R, T

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS-II BEAMLINE 17-ID-2
Spacegroup: P21
Unit cell:
a: 113.21Å b: 123.69Å c: 136.909Å
α: 90° β: 100.36° γ: 90°
R-values:
R R work R free
0.204 0.2 0.283
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided
  • Homo sapiens

Quick links

Citations

79 review citations

Tumor suppressor p53: Biology, signaling pathways, and therapeutic targeting.
Hernández Borrero et al. (2021)
78 more

4 mentions without citation

Structural basis for T cell recognition of cancer neoantigens and implications for predicting neoepitope immunogenicity.
Mariuzza et al. (2023)
3 more