6w5c

Electron Microscopy
2.9Å resolution

Cryo-EM structure of Cas12i(E894A)-crRNA-dsDNA complex

Released:
Primary publication:
Mechanisms for target recognition and cleavage by the Cas12i RNA-guided endonuclease.
Nat Struct Mol Biol 27 1069-1076 (2020)
PMID: 32895556
Related structures: EMD-21541

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-120806 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Cas12i Chain: A
Molecule details ›
Chain: A
Length: 1092 amino acids
Theoretical weight: 125.72 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli BL21(DE3)
TS Chain: C
Molecule details ›
Chain: C
Length: 36 nucleotides
Theoretical weight: 11.08 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli BL21(DE3)
NTS Chain: D
Molecule details ›
Chain: D
Length: 10 nucleotides
Theoretical weight: 2.97 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli BL21(DE3)
Substrate Chain: E
Molecule details ›
Chain: E
Length: 9 nucleotides
Theoretical weight: 2.79 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli BL21(DE3)
crRNA Chain: B
Molecule details ›
Chain: B
Length: 59 nucleotides
Theoretical weight: 18.86 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-21541
Expression system: Escherichia coli BL21(DE3)