› 6w64

Electron Microscopy
3.9Å resolution

Cryo-EM structure of Cas12i-crRNA-dsDNA complex in I1 state

Released:
DOI: 10.2210/pdb6w64/pdb
PDB entry 6w64 coloured by chain, front view.
PDB entry 6w64 coloured by chain, side view.
PDB entry 6w64 coloured by chain, top view.
Source organism: Lachnospiraceae bacterium ND2006
Primary publication:
Mechanisms for target recognition and cleavage by the Cas12i RNA-guided endonuclease.
Zhang H, Li Z, Xiao R, Chang L
Nat Struct Mol Biol 27 1069-1076 (2020)
PMID: 32895556
Related structures: EMD-21552

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-120811 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
Cas12i Chain: A
Molecule details ›
Chain: A
Length: 1092 amino acids
Theoretical weight: 125.72 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli BL21(DE3)
DNA (25-MER) Chain: C
Molecule details ›
Chain: C
Length: 25 nucleotides
Theoretical weight: 7.71 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli BL21(DE3)
crRNA Chain: B
Molecule details ›
Chain: B
Length: 38 nucleotides
Theoretical weight: 12.15 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-21552
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Discovery of Diverse CRISPR-Cas Systems and Expansion of the Genome Engineering Toolbox.
Koonin et al. (2023)
3 more

1 mention without citation

Mechanisms for target recognition and cleavage by the Cas12i RNA-guided endonuclease.
Zhang et al. (2020)