› 6w65

X-ray diffraction
2.13Å resolution

Human PARP16 in complex with RBN010860

Released:
DOI: 10.2210/pdb6w65/pdb
PDB entry 6w65 coloured by chain, front view.
PDB entry 6w65 coloured by chain, side view.
PDB entry 6w65 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
In Vitro and Cellular Probes to Study PARP Enzyme Target Engagement.
Wigle TJ, Blackwell DJ, Schenkel LB, Ren Y, Church WD, Desai HJ, Swinger KK, Santospago AG, Majer CR, Lu AZ, Niepel M, Perl NR, Vasbinder MM, Keilhack H, Kuntz KW
Cell Chem Biol 27 877-887.e14 (2020)
PMID: 32679093

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185408 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein mono-ADP-ribosyltransferase PARP16 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 277 amino acids
Theoretical weight: 31.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N5Y8 (Residues: 5-279; Coverage: 85%)
Gene names: ARTD15, C15orf30, PARP16
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand T9D 3 x T9D
Ligand CIT 2 x CIT
Ligand GOL 10 x GOL
Ligand EDO 4 x EDO
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P43212
Unit cell:
a: 147.12Å b: 147.12Å c: 99.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.225
Expression system: Escherichia coli

Quick links

Citations

7 review citations

MARTs and MARylation in the Cytosol: Biological Functions, Mechanisms of Action, and Therapeutic Potential.
Challa et al. (2021)
6 more