› 6w6n

X-ray diffraction
2.25Å resolution

K106L/A131E mutant of cytochrome P460 from Nitrosomonas sp. AL212

Released:
DOI: 10.2210/pdb6w6n/pdb
PDB entry 6w6n coloured by chain, front view.
PDB entry 6w6n coloured by chain, side view.
PDB entry 6w6n coloured by chain, top view.
Source organism: Nitrosomonas sp. AL212
Primary publication:
The Heme-Lys Cross-Link in Cytochrome P460 Promotes Catalysis by Enforcing Secondary Coordination Sphere Architecture.
Coleman RE, Vilbert AC, Lancaster KM
Biochemistry 59 2289-2298 (2020)
PMID: 32525655

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P460 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 160 amino acids
Theoretical weight: 17.91 KDa
Source organism: Nitrosomonas sp. AL212
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F9ZFJ0 (Residues: 35-194; Coverage: 95%)
Gene name: NAL212_0896
Sequence domains: Cytochrome P460

Ligands and Environments

1 bound ligand:
Ligand HEC 2 x HEC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 47.373Å b: 80.172Å c: 110.767Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.258 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

A novel catalytic heme cofactor in SfmD with a single thioether bond and a bis-His ligand set revealed by a de novo crystal structural and spectroscopic study.
Shin et al. (2021)
2 more

2 mentions without citation

Heme P460: A (Cross) Link to Nitric Oxide.
Coleman et al. (2020)
1 more