› 6w6w

Electron Microscopy
3Å resolution

Cryo-EM structure of CST bound to telomeric single-stranded DNA

Released:
DOI: 10.2210/pdb6w6w/pdb
PDB entry 6w6w coloured by chain, front view.
PDB entry 6w6w coloured by chain, side view.
PDB entry 6w6w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of human CST reveals a decameric assembly bound to telomeric DNA.
Lim CJ, Barbour AT, Zaug AJ, Goodrich KJ, McKay AE, Wuttke DS, Cech TR
Science 368 1081-1085 (2020)
PMID: 32499435
Related structures: EMD-21567

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120812 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (4 distinct):
CST complex subunit CTC1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1233 amino acids
Theoretical weight: 136.75 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q2NKJ3 (Residues: 2-1217; Coverage: 100%)
Gene names: C17orf68, CTC1
Sequence domains: CST, telomere maintenance, complex subunit CTC1
CST complex subunit STN1 Chain: C
Molecule details ›
Chain: C
Length: 374 amino acids
Theoretical weight: 43 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9H668 (Residues: 2-368; Coverage: 100%)
Gene names: OBFC1, STN1
Sequence domains:
CST complex subunit TEN1 Chain: D
Molecule details ›
Chain: D
Length: 152 amino acids
Theoretical weight: 17.29 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q86WV5 (Residues: 3-123; Coverage: 98%)
Gene names: C17orf106, TEN1
Sequence domains: Telomere-capping, CST complex subunit
DNA (5'-D(P*TP*AP*GP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 4 nucleotides
Theoretical weight: 1.23 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-21567
Expression system: Trichoplusia ni

Quick links

Citations

18 review citations

Roles of OB-Fold Proteins in Replication Stress.
Nguyen et al. (2020)
17 more

9 mentions without citation

CST in maintaining genome stability: Beyond telomeres.
Lyu et al. (2021)
8 more