› 6w7w

Electron Microscopy
3.9Å resolution

30S-Inactive-low-Mg2+ Class B

Released:
DOI: 10.2210/pdb6w7w/pdb
PDB entry 6w7w coloured by chain, front view.
PDB entry 6w7w coloured by chain, side view.
PDB entry 6w7w coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Alternative conformations and motions adopted by 30S ribosomal subunits visualized by cryo-electron microscopy.
Jahagirdar D, Jha V, Basu K, Gomez-Blanco J, Vargas J, Ortega J
RNA 26 2017-2030 (2020)
PMID: 32989043
Related structures: EMD-21573

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135742 (preferred)
Entry contents:
9 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (10 distinct):
Small ribosomal subunit protein uS4 Chain: C
Molecule details ›
Chain: C
Length: 206 amino acids
Theoretical weight: 23.51 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7V8 (Residues: 1-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Sequence domains:
Small ribosomal subunit protein uS5 Chain: D
Molecule details ›
Chain: D
Length: 167 amino acids
Theoretical weight: 17.63 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7W1 (Residues: 1-167; Coverage: 100%)
Gene names: JW3265, b3303, rpsE, spc
Sequence domains:
Small ribosomal subunit protein bS6 Chain: E
Molecule details ›
Chain: E
Length: 135 amino acids
Theoretical weight: 15.73 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P02358 (Residues: 1-135; Coverage: 100%)
Gene names: JW4158, b4200, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS8 Chain: G
Molecule details ›
Chain: G
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7W7 (Residues: 1-130; Coverage: 100%)
Gene names: JW3268, b3306, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS12 Chain: K
Molecule details ›
Chain: K
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7S3 (Residues: 1-124; Coverage: 100%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS15 Chain: N
Molecule details ›
Chain: N
Length: 89 amino acids
Theoretical weight: 10.32 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0ADZ4 (Residues: 1-89; Coverage: 100%)
Gene names: JW3134, b3165, rpsO, secC
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: O
Molecule details ›
Chain: O
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Gene names: JW2590, b2609, rpsP
Sequence domains: Ribosomal protein S16
Small ribosomal subunit protein uS17 Chain: P
Molecule details ›
Chain: P
Length: 84 amino acids
Theoretical weight: 9.72 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0AG63 (Residues: 1-84; Coverage: 100%)
Gene names: JW3273, b3311, neaA, rpsQ
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein bS20 Chain: S
Molecule details ›
Chain: S
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7U7 (Residues: 1-87; Coverage: 100%)
Gene names: JW0022, b0023, rpsT
Sequence domains: Ribosomal protein S20
16S ribosomal RNA Chain: 2
Molecule details ›
Chain: 2
Length: 1542 nucleotides
Theoretical weight: 499.69 KDa
Sequence domains: Bacterial small subunit ribosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-21573

Quick links

Citations

2 review citations

The Structural Dynamics of Translation.
Korostelev AA. (2022)
1 more