› 6w8w

X-ray diffraction
3Å resolution

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120819 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA (cytosine-5)-methyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 873 amino acids
Theoretical weight: 98.98 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13864 (Residues: 731-1602; Coverage: 54%)
Gene names: Dnmt, Dnmt1, Met1, Uim
Sequence domains:
CCG DNA (5'-D(*AP*CP*TP*TP*AP*(5CM)P*GP*GP*AP*AP*GP*G)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 12 nucleotides
Theoretical weight: 3.73 KDa
Source organism: synthetic construct
Expression system: Not provided
CCG DNA (5'-D(*CP*CP*TP*TP*CP*(C49)P*GP*TP*AP*AP*GP*T)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
Ligand ZN 4 x ZN
2 modified residues:
Ligand 5CM 2 x 5CM
Ligand C49 2 x C49

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 89.794Å b: 152.593Å c: 95.631Å
α: 90° β: 94.74° γ: 90°
R-values:
R R work R free
0.24 0.238 0.28
Expression systems:
  • Escherichia coli
  • Not provided

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