› 6wc2

X-ray diffraction
2.1Å resolution

Crystal Structure of a Ternary MEF2 Chimera/NKX2-5/myocardin enhancer DNA Complex

Released:
DOI: 10.2210/pdb6wc2/pdb
PDB entry 6wc2 coloured by chain, front view.
PDB entry 6wc2 coloured by chain, side view.
PDB entry 6wc2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structures of Ternary Complexes of MEF2 and NKX2-5 Bound to DNA Reveal a Disease Related Protein-Protein Interaction Interface.
Lei X, Zhao J, Sagendorf JM, Rajashekar N, Xu J, Dantas Machado AC, Sen C, Rohs R, Feng P, Chen L
J Mol Biol 432 5499-5508 (2020)
PMID: 32681840

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120822 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Myocyte-specific enhancer factor 2A; Myocyte-specific enhancer factor 2B Chains: A, B, C, D, I, J
Molecule details ›
Chains: A, B, C, D, I, J
Length: 95 amino acids
Theoretical weight: 11.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02080 (Residues: 1-8, 73-91; Coverage: 7%)
  • Canonical: Q02078 (Residues: 9-72, 92-95; Coverage: 13%)
Gene names: MEF2, MEF2A, MEF2B, XMEF2
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Homeobox protein Nkx-2.5 Chains: M, N, O
Molecule details ›
Chains: M, N, O
Length: 61 amino acids
Theoretical weight: 7.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52952 (Residues: 137-197; Coverage: 19%)
Gene names: CSX, NKX2-5, NKX2.5, NKX2E
Sequence domains: Homeodomain
Myocardin Enhancer DNA Chains: E, G, K
Molecule details ›
Chains: E, G, K
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
Myocardin Enhancer DNA Chains: F, H, L
Molecule details ›
Chains: F, H, L
Length: 21 nucleotides
Theoretical weight: 6.44 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 69.37Å b: 133.9Å c: 140.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.225
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

The benign nature and rare occurrence of cardiac myxoma as a possible consequence of the limited cardiac proliferative/ regenerative potential: a systematic review.
Shafi et al. (2023)

1 mention without citation

Crystal Structures of Ternary Complexes of MEF2 and NKX2-5 Bound to DNA Reveal a Disease Related Protein-Protein Interaction Interface.
Lei et al. (2020)