› 6wcq

Electron Microscopy
8.5Å resolution

Structure of a substrate-bound DQC ubiquitin ligase

Released:
DOI: 10.2210/pdb6wcq/pdb
PDB entry 6wcq coloured by chain, front view.
PDB entry 6wcq coloured by chain, side view.
PDB entry 6wcq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for dimerization quality control.
Mena EL, Jevtić P, Greber BJ, Gee CL, Lew BG, Akopian D, Nogales E, Kuriyan J, Rape M
Nature 586 452-456 (2020)
PMID: 32814905
Related structures: EMD-21617

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-159038 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
S-phase kinase-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.68 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63208 (Residues: 1-163; Coverage: 100%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
F-box/LRR-repeat protein 17 Chain: B
Molecule details ›
Chain: B
Length: 396 amino acids
Theoretical weight: 44.97 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9UF56 (Residues: 310-701; Coverage: 56%)
Gene names: FBL17, FBX13, FBXL17, FBXO13
Sequence domains:
Kelch-like ECH-associated protein 1 Chain: C
Molecule details ›
Chain: C
Length: 631 amino acids
Theoretical weight: 70.17 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q14145 (Residues: 1-624; Coverage: 100%)
Gene names: INRF2, KEAP1, KIAA0132, KLHL19
Sequence domains:
Cullin-1 Chain: D
Molecule details ›
Chain: D
Length: 434 amino acids
Theoretical weight: 49.93 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q13616 (Residues: 1-434; Coverage: 56%)
Gene name: CUL1
Sequence domains: Cullin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.5Å
Relevant EMDB volumes: EMD-21617
Expression system: Trichoplusia ni

Quick links

Citations

2 review citations

Cullin-RING Ubiquitin Ligase Regulatory Circuits: A Quarter Century Beyond the F-Box Hypothesis.
Harper et al. (2021)
1 more

3 mentions without citation

Structural basis for dimerization quality control.
Mena et al. (2020)
2 more