› 6wcx

X-ray diffraction
2.89Å resolution

FphF, Staphylococcus aureus fluorophosphonate-binding serine hydrolases F, substrate bound

Released:
DOI: 10.2210/pdb6wcx/pdb
PDB entry 6wcx coloured by chain, front view.
PDB entry 6wcx coloured by chain, side view.
PDB entry 6wcx coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural Basis for the Inhibitor and Substrate Specificity of the Unique Fph Serine Hydrolases of Staphylococcus aureus.
Fellner M, Lentz CS, Jamieson SA, Brewster JL, Chen L, Bogyo M, Mace PD
ACS Infect Dis 6 2771-2782 (2020)
PMID: 32865965

Function and Biology Details

Reaction catalysed: 
S-formylglutathione + H(2)O = glutathione + formate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-formylglutathione hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 255 amino acids
Theoretical weight: 29.21 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2FUY3 (Residues: 2-253; Coverage: 100%)
Gene name: SAOUHSC_02962
Sequence domains: Putative esterase

Ligands and Environments

1 bound ligand:
Ligand HE4 4 x HE4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P6122
Unit cell:
a: 86.957Å b: 86.957Å c: 454.712Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.225 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

From Differential Stains to Next Generation Physiology: Chemical Probes to Visualize Bacterial Cell Structure and Physiology.
Hira et al. (2020)
1 more

2 mentions without citation

Newly discovered Staphylococcus aureus serine hydrolase probe and drug targets.
Fellner M. (2022)
1 more