› 6wef

X-ray diffraction
2.5Å resolution

Copper-bound D92H variant of Campylobacter jejuni P19

Released:
DOI: 10.2210/pdb6wef/pdb
PDB entry 6wef coloured by chain, front view.
PDB entry 6wef coloured by chain, side view.
PDB entry 6wef coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni 81-176
Primary publication:
A copper site is required for iron transport by the periplasmic proteins P19 and FetP.
Chan ACK, Lin H, Koch D, Grass G, Nies DH, Murphy MEP
Metallomics 12 1530-1541 (2020)
PMID: 32780051

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron transporter Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 159 amino acids
Theoretical weight: 17.61 KDa
Source organism: Campylobacter jejuni subsp. jejuni 81-176
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3PA01 (Residues: 22-179; Coverage: 100%)
Gene name: CJJ81176_1650
Sequence domains: Fe2+ transport protein

Ligands and Environments

3 bound ligands:
Ligand CU 12 x CU
Ligand SO4 11 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P32
Unit cell:
a: 132.455Å b: 132.455Å c: 104.585Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.272 0.27 0.299
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Dissecting components of the Campylobacter jejuni fetMP-fetABCDEF gene cluster under iron limitation.
Richardson-Sanchez et al. (2024)