› 6wf0

X-ray diffraction
3.46Å resolution

Crystal Structure of Broadly Neutralizing Antibody 3I14 Bound to the Influenza A H3 Hemagglutinin

Released:
DOI: 10.2210/pdb6wf0/pdb
Model geometry
Fit model/data
PDB entry 6wf0 coloured by chain, front view.
PDB entry 6wf0 coloured by chain, side view.
PDB entry 6wf0 coloured by chain, top view.
Source organisms:
Primary publication:
Unique structural solution from a VH3-30 antibody targeting the hemagglutinin stem of influenza A viruses.
Harshbarger WD, Deming D, Lockbaum GJ, Attatippaholkun N, Kamkaew M, Hou S, Somasundaran M, Wang JP, Finberg RW, Zhu QK, Schiffer CA, Marasco WA
OpenAccess logo Nat Commun 12 559 (2021)
PMID: 33495478

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-282627 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 35.23 KDa
Source organism: Influenza A virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A5P1MU07 (Residues: 27-341; Coverage: 56%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin Chain: B
Molecule details ›
Chain: B
Length: 163 amino acids
Theoretical weight: 18.92 KDa
Source organism: Influenza A virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A2P1E3C0 (Residues: 339-501; Coverage: 30%)
Gene name: HA
Sequence domains: Haemagglutinin
heavy chain Chain: H
light chain Chain: L

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P63
Unit cell:
a: 130.318Å b: 130.318Å c: 188.552Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.231 0.282
Expression system: Trichoplusia ni

Quick links

Citations

2 review citations

Antibodies to combat viral infections: development strategies and progress.
Pantaleo et al. (2022)
1 more

3 mentions without citation

Analysis of the conserved protective epitopes of hemagglutinin on influenza A viruses.
Jiao et al. (2023)
2 more