6wg3

Electron Microscopy
5.3Å resolution

Cryo-EM structure of human Cohesin-NIPBL-DNA complex

Released:
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of the human cohesin-NIPBL-DNA complex.
Science 368 1454-1459 (2020)
PMID: 32409525
Related structures: EMD-21658

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-120834 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Structural maintenance of chromosomes protein 1A Chain: A
Molecule details ›
Chain: A
Length: 1233 amino acids
Theoretical weight: 143.48 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q14683 (Residues: 1-1233; Coverage: 100%)
Gene names: DXS423E, KIAA0178, SB1.8, SMC1, SMC1A, SMC1L1
Sequence domains:
Structural maintenance of chromosomes protein 3 Chain: B
Molecule details ›
Chain: B
Length: 1217 amino acids
Theoretical weight: 141.77 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9UQE7 (Residues: 1-1217; Coverage: 100%)
Gene names: BAM, BMH, CSPG6, SMC3, SMC3L1
Sequence domains:
Double-strand-break repair protein rad21 homolog Chain: C
Molecule details ›
Chain: C
Length: 631 amino acids
Theoretical weight: 71.56 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O60216 (Residues: 1-631; Coverage: 100%)
Gene names: HR21, KIAA0078, NXP1, RAD21, SCC1
Sequence domains:
Cohesin subunit SA-1 Chain: D
Molecule details ›
Chain: D
Length: 1272 amino acids
Theoretical weight: 146.08 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8WVM7 (Residues: 1-1258; Coverage: 100%)
Gene names: SA1, SCC3, STAG1
Sequence domains:
Nipped-B-like protein Chain: E
Molecule details ›
Chain: E
Length: 1652 amino acids
Theoretical weight: 188.15 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6KC79 (Residues: 1163-2804; Coverage: 59%)
Gene names: IDN3, NIPBL, SCC2
Sequence domains:
DNA (51-MER) Chain: F
Molecule details ›
Chain: F
Length: 51 nucleotides
Theoretical weight: 15.93 KDa
DNA (51-MER) Chain: G
Molecule details ›
Chain: G
Length: 51 nucleotides
Theoretical weight: 15.47 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.3Å
Relevant EMDB volumes: EMD-21658
Expression system: Trichoplusia ni