6wgc

Electron Microscopy
4.3Å resolution

Atomic model of semi-attached mutant OCCM-DNA complex (ORC-Cdc6-Cdt1-Mcm2-7 with Mcm6 WHD truncation)

Released:
Source organism: Saccharomyces cerevisiae
Related structures: EMD-21662

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-120837 (preferred)
Entry contents:
9 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (11 distinct):
Cell division control protein 6 Chain: 9
Molecule details ›
Chain: 9
Length: 513 amino acids
Theoretical weight: 58.11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P09119 (Residues: 1-513; Coverage: 100%)
Gene names: CDC6, J0347, YJL194W
Sequence domains: AAA domain
Origin recognition complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 913 amino acids
Theoretical weight: 104.43 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P54784 (Residues: 1-913; Coverage: 100%)
Gene names: ORC1, YML065W
Sequence domains:
Origin recognition complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 620 amino acids
Theoretical weight: 71.34 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32833 (Residues: 1-620; Coverage: 100%)
Gene names: ORC2, RRR1, SIR5, YBR0523, YBR060C
Sequence domains: Origin recognition complex subunit 2
Origin recognition complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 616 amino acids
Theoretical weight: 72.16 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P54790 (Residues: 1-616; Coverage: 100%)
Gene names: L1365, OAF1, OIF1, ORC3, YLL004W
Sequence domains: Origin recognition complex winged helix C-terminal
Origin recognition complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 479 amino acids
Theoretical weight: 55.35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P50874 (Residues: 1-479; Coverage: 100%)
Gene names: N0834, ORC5, YNL261W
Sequence domains:
Origin recognition complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 529 amino acids
Theoretical weight: 60.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P54791 (Residues: 1-529; Coverage: 100%)
Gene names: ORC4, P9325.5, YPR162C
Sequence domains: Origin recognition complex (ORC) subunit 4 C-terminus
Origin recognition complex subunit 6 Chain: F
Molecule details ›
Chain: F
Length: 435 amino acids
Theoretical weight: 50.37 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38826 (Residues: 1-435; Coverage: 100%)
Gene names: AAP1, ORC6, YHR118C
Sequence domains: Origin recognition complex subunit 6 (ORC6)
DNA replication licensing factor MCM3 Chain: 3
Molecule details ›
Chain: 3
Length: 971 amino acids
Theoretical weight: 107.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P24279 (Residues: 1-971; Coverage: 100%)
Gene names: MCM3, SYGP-ORF23, YEL032W
Sequence domains:
DNA replication licensing factor MCM7 Chain: 7
Molecule details ›
Chain: 7
Length: 845 amino acids
Theoretical weight: 95.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38132 (Residues: 1-845; Coverage: 100%)
Gene names: CDC47, MCM7, YBR1441, YBR202W
Sequence domains:
DNA (41-MER) Chain: G
Molecule details ›
Chain: G
Length: 41 nucleotides
Theoretical weight: 12.58 KDa
DNA (41-MER) Chain: H
Molecule details ›
Chain: H
Length: 41 nucleotides
Theoretical weight: 12.66 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-21662
Expression system: Saccharomyces cerevisiae