› 6wge

Electron Microscopy
3.9Å resolution

Cryo-EM structure of human Cohesin-NIPBL-DNA complex without STAG1

Released:
DOI: 10.2210/pdb6wge/pdb
PDB entry 6wge coloured by chain, front view.
PDB entry 6wge coloured by chain, side view.
PDB entry 6wge coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of the human cohesin-NIPBL-DNA complex.
Shi Z, Gao H, Bai XC, Yu H
Science 368 1454-1459 (2020)
PMID: 32409525
Related structures: EMD-21663

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-120838 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
Structural maintenance of chromosomes protein 1A Chain: A
Molecule details ›
Chain: A
Length: 1233 amino acids
Theoretical weight: 143.48 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q14683 (Residues: 1-1233; Coverage: 100%)
Gene names: DXS423E, KIAA0178, SB1.8, SMC1, SMC1A, SMC1L1
Sequence domains:
Structural maintenance of chromosomes protein 3 Chain: B
Molecule details ›
Chain: B
Length: 1217 amino acids
Theoretical weight: 141.77 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9UQE7 (Residues: 1-1217; Coverage: 100%)
Gene names: BAM, BMH, CSPG6, SMC3, SMC3L1
Sequence domains:
Double-strand-break repair protein rad21 homolog Chain: C
Molecule details ›
Chain: C
Length: 631 amino acids
Theoretical weight: 71.56 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O60216 (Residues: 1-631; Coverage: 100%)
Gene names: HR21, KIAA0078, NXP1, RAD21, SCC1
Sequence domains:
Nipped-B-like protein Chain: E
Molecule details ›
Chain: E
Length: 1642 amino acids
Theoretical weight: 186.78 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6KC79 (Residues: 1163-2804; Coverage: 59%)
Gene names: IDN3, NIPBL, SCC2
Sequence domains:
DNA (43-MER) Chain: F
Molecule details ›
Chain: F
Length: 43 nucleotides
Theoretical weight: 13.42 KDa
DNA (43-MER) Chain: G
Molecule details ›
Chain: G
Length: 43 nucleotides
Theoretical weight: 13.04 KDa

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-21663
Expression system: Trichoplusia ni

Quick links

Citations

20 review citations

Role of Structural and Non-Structural Proteins and Therapeutic Targets of SARS-CoV-2 for COVID-19.
Yadav et al. (2021)
19 more

4 mentions without citation

Comprehensive review and expanding the genetic landscape of Cornelia-de-Lange spectrum: insights from novel mutations and skin biopsy in exome-negative cases.
Tehrani Fateh et al. (2024)
3 more