6whb

X-ray diffraction
1.9Å resolution

MEKK1 TOG domain (548-867)

Released:
Source organism: Homo sapiens
Primary publication:
A cryptic tubulin-binding domain links MEKK1 to curved tubulin protomers.
Proc Natl Acad Sci U S A 117 21308-21318 (2020)
PMID: 32817551

Function and Biology Details

Reactions catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-171612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase kinase kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 37.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13233 (Residues: 542-888; Coverage: 23%)
Gene names: MAP3K1, MAPKKK1, MEKK, MEKK1
Sequence domains: Centrosomal protein CEP104-like, TOG domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: I4122
Unit cell:
a: 71.817Å b: 71.817Å c: 259.561Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.23
Expression system: Escherichia coli BL21(DE3)