› 6whi

Electron Microscopy
4.2Å resolution

Cryo-electron microscopy structure of the type I-F CRISPR RNA-guided surveillance complex bound to the anti-CRISPR AcrIF9

Released:
DOI: 10.2210/pdb6whi/pdb
PDB entry 6whi coloured by chain, front view.
PDB entry 6whi coloured by chain, side view.
PDB entry 6whi coloured by chain, top view.
Source organisms:
Primary publication:
AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex.
Hirschi M, Lu WT, Santiago-Frangos A, Wilkinson R, Golden SM, Davidson AR, Lander GC, Wiedenheft B
Nat Commun 11 2730 (2020)
PMID: 32483187
Related structures: EMD-21517

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
PDBe Complex ID:
PDB-CPX-236848 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
CRISPR-associated protein Csy1 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 49.19 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02ML9 (Residues: 1-434; Coverage: 100%)
Gene names: PA14_33330, csy1
Sequence domains: CRISPR-associated protein (Cas_Csy1)
CRISPR-associated protein Csy2 Chain: B
Molecule details ›
Chain: B
Length: 327 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated protein Csy3 Chains: C, D, E, F, G, H
Molecule details ›
Chains: C, D, E, F, G, H
Length: 360 amino acids
Theoretical weight: 39.78 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02MM1 (Residues: 2-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
CRISPR-associated endonuclease Cas6/Csy4 Chain: L
Molecule details ›
Chain: L
Length: 187 amino acids
Theoretical weight: 21.43 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02MM2 (Residues: 1-187; Coverage: 100%)
Gene names: PA14_33300, cas6f, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
anti-CRISPR AcrIF9 Chains: I, J
Molecule details ›
Chains: I, J
Length: 68 amino acids
Theoretical weight: 7.86 KDa
Source organism: Proteus penneri
Expression system: Escherichia coli
non-target dsDNA Chains: N, Q
Molecule details ›
Chains: N, Q
Length: 77 nucleotides
Theoretical weight: 23.8 KDa
non-target dsDNA Chains: O, P
Molecule details ›
Chains: O, P
Length: 77 nucleotides
Theoretical weight: 23.69 KDa
RNA (60-MER) Chain: M
Molecule details ›
Chain: M
Length: 60 nucleotides
Theoretical weight: 19.27 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-21517
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins.
Jia et al. (2021)
4 more

1 mention without citation

Structural insights into the inactivation of the type I-F CRISPR-Cas system by anti-CRISPR proteins.
Yang et al. (2021)