› 6wia

X-ray diffraction
2.21Å resolution

CRYSTAL STRUCTURE OF HUMAN PROTECTIVE PROTEIN/CATHEPSIN A, DFP-INHIBITED (AGED)

Released:
DOI: 10.2210/pdb6wia/pdb
PDB entry 6wia coloured by chain, front view.
PDB entry 6wia coloured by chain, side view.
PDB entry 6wia coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and kinetic evidence of aging after organophosphate inhibition of human Cathepsin A.
Bouknight KD, Jurkouich KM, Compton JR, Khavrutskii IV, Guelta MA, Harvey SP, Legler PM
Biochem Pharmacol 177 113980 (2020)
PMID: 32305437

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal amino acid with broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145363 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysosomal protective protein Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 52.41 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P10619 (Residues: 29-480; Coverage: 100%)
Gene names: CTSA, PPGB
Sequence domains: Serine carboxypeptidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand ACT 5 x ACT
Ligand CD 6 x CD
Ligand GOL 1 x GOL
1 modified residue:
Ligand MIS 1 x MIS

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: C2
Unit cell:
a: 91.273Å b: 101.999Å c: 48.114Å
α: 90° β: 101.61° γ: 90°
R-values:
R R work R free
0.196 0.195 0.224
Expression system: Homo sapiens

Quick links

Citations

2 citation in other articles

In Silico Structural Analysis of Serine Carboxypeptidase Nf314, a Potential Drug Target in Naegleria fowleri Infections.
Madero-Ayala et al. (2022)
1 more

1 mention without citation

Structure-function analysis for the development of peptide inhibitors for a Gram-positive quorum sensing system.
Abdullah et al. (2022)