› 6wig

X-ray diffraction
2.1Å resolution

Structure of STENOFOLIA Protein HD domain bound with DNA

Released:
DOI: 10.2210/pdb6wig/pdb
PDB entry 6wig coloured by chain, front view.
PDB entry 6wig coloured by chain, side view.
PDB entry 6wig coloured by chain, top view.
Source organism: Medicago truncatula
Primary publication:
Structure of the unique tetrameric STENOFOLIA homeodomain bound with target promoter DNA.
Pathak PK, Zhang F, Peng S, Niu L, Chaturvedi J, Elliott J, Xiang Y, Tadege M, Deng J
Acta Crystallogr D Struct Biol 77 1050-1063 (2021)
PMID: 34342278

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120844 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homeobox domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 12.37 KDa
Source organism: Medicago truncatula
Expression system: Escherichia coli
UniProt:
  • Canonical: G0ZGT3 (Residues: 85-190; Coverage: 30%)
Gene name: STF
Sequence domains: Homeodomain
DNA (5'-D(P*GP*CP*AP*AP*AP*TP*TP*AP*AP*TP*GP*AP*TP*TP*TP*AP*TP*TP*CP*AP*AP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 22 nucleotides
Theoretical weight: 6.77 KDa
Source organism: Medicago truncatula
Expression system: Not provided
DNA (5'-D(P*CP*TP*TP*GP*AP*AP*TP*AP*AP*AP*TP*CP*AP*TP*TP*AP*AP*TP*TP*TP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 22 nucleotides
Theoretical weight: 6.72 KDa
Source organism: Medicago truncatula
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 48.061Å b: 49.489Å c: 69.81Å
α: 90° β: 106.46° γ: 90°
R-values:
R R work R free
0.2 0.194 0.25
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 mention without citation

Structure of the unique tetrameric STENOFOLIA homeodomain bound with target promoter DNA.
Pathak et al. (2021)