› 6wl4

X-ray diffraction
3.6Å resolution

preTCRbeta-pMHC complex crystal structure

Released:
DOI: 10.2210/pdb6wl4/pdb
PDB entry 6wl4 coloured by chain, front view.
PDB entry 6wl4 coloured by chain, side view.
PDB entry 6wl4 coloured by chain, top view.
Source organisms:
Primary publication:
Pre-T cell receptors topologically sample self-ligands during thymocyte β-selection.
Li X, Mizsei R, Tan K, Mallis RJ, Duke-Cohan JS, Akitsu A, Tetteh PW, Dubey A, Hwang W, Wagner G, Lang MJ, Arthanari H, Wang JH, Reinherz EL
Science 371 181-185 (2021)
PMID: 33335016

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-208746 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chains: A, D, G
Molecule details ›
Chains: A, D, G
Length: 185 amino acids
Theoretical weight: 21.32 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01901 (Residues: 22-206; Coverage: 53%)
Gene names: H2-K, H2-K1
Sequence domains: Class I Histocompatibility antigen, domains alpha 1 and 2
Nucleoprotein Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 8 amino acids
Theoretical weight: 956 Da
Source organism: Vesicular stomatitis virus
Expression system: Not provided
UniProt:
  • Canonical: P03521 (Residues: 52-59; Coverage: 2%)
Gene name: N
N15 preTCR beta Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 239 amino acids
Theoretical weight: 27.35 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 110.546Å b: 110.546Å c: 316.917Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.269
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

The perception and response of T cells to a changing environment are based on the law of initial value.
Huseby et al. (2022)
3 more

1 mention without citation

Pre-T cell receptors topologically sample self-ligands during thymocyte β-selection.
Li et al. (2021)