› 6wmo

X-ray diffraction
1.85Å resolution

Human poly-N-acetyl-lactosamine synthase structure demonstrates a modular assembly of catalytic subsites for GT-A glycosyltransferases

Released:
DOI: 10.2210/pdb6wmo/pdb
PDB entry 6wmo coloured by chain, front view.
PDB entry 6wmo coloured by chain, side view.
PDB entry 6wmo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Comparison of human poly-N-acetyl-lactosamine synthase structure with GT-A fold glycosyltransferases supports a modular assembly of catalytic subsites.
Kadirvelraj R, Yang JY, Kim HW, Sanders JH, Moremen KW, Wood ZA
J Biol Chem 296 100110
PMID: 33229435

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 364 amino acids
Theoretical weight: 42.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NY97 (Residues: 35-397; Coverage: 91%)
Gene names: B3GALT7, B3GNT1, B3GNT2
Sequence domains: Galactosyltransferase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
5 bound ligands:
Ligand MG 2 x MG
Ligand UDP 2 x UDP
Ligand NAG 3 x NAG
Ligand EDO 5 x EDO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 48.19Å b: 109.28Å c: 147.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.212
Expression system: Homo sapiens

Quick links

Citations

6 citation in other articles

A photo-cross-linking GlcNAc analog enables covalent capture of N-linked glycoprotein-binding partners on the cell surface.
Wu et al. (2022)
5 more

4 mentions without citation

Comparison of human poly-N-acetyl-lactosamine synthase structure with GT-A fold glycosyltransferases supports a modular assembly of catalytic subsites.
Kadirvelraj et al. (2021)
3 more