› 6wmp

Electron Microscopy
2.98Å resolution

F. tularensis RNAPs70-iglA DNA complex

Released:
DOI: 10.2210/pdb6wmp/pdb
PDB entry 6wmp coloured by chain, front view.
PDB entry 6wmp coloured by chain, side view.
PDB entry 6wmp coloured by chain, top view.
Source organism: Francisella tularensis subsp. holarctica LVS
Primary publication:
Structural Basis for Virulence Activation of Francisella tularensis.
Travis BA, Ramsey KM, Prezioso SM, Tallo T, Wandzilak JM, Hsu A, Borgnia M, Bartesaghi A, Dove SL, Brennan RG, Schumacher MA
Mol Cell 81 139-152.e10 (2021)
PMID: 33217319
Related structures: EMD-21850

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120853 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha 1 Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.39 KDa
Source organism: Francisella tularensis subsp. holarctica LVS
UniProt:
  • Canonical: Q2A5E5 (Residues: 1-323; Coverage: 100%)
Gene names: FTL_0261, rpoA1
Sequence domains:
DNA-directed RNA polymerase subunit alpha 2 Chain: B
Molecule details ›
Chain: B
Length: 317 amino acids
Theoretical weight: 35.11 KDa
Source organism: Francisella tularensis subsp. holarctica LVS
UniProt:
  • Canonical: Q2A4H7 (Residues: 1-317; Coverage: 100%)
Gene names: FTL_0616, rpoA2
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1358 amino acids
Theoretical weight: 151.54 KDa
Source organism: Francisella tularensis subsp. holarctica LVS
UniProt:
  • Canonical: Q2A1M7 (Residues: 1-1358; Coverage: 100%)
Gene names: FTL_1744, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1604 amino acids
Theoretical weight: 178.45 KDa
Source organism: Francisella tularensis subsp. holarctica LVS
UniProt:
  • Canonical: Q2A1M8 (Residues: 1-1417; Coverage: 100%)
Gene names: FTL_1743, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 72 amino acids
Theoretical weight: 8.18 KDa
Source organism: Francisella tularensis subsp. holarctica LVS
UniProt:
  • Canonical: Q2A273 (Residues: 1-72; Coverage: 100%)
Gene names: FTL_1533, rpoZ
Sequence domains: RNA polymerase Rpb6
DNA NT-strand Chain: F
Molecule details ›
Chain: F
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
DNA T-strand Chain: G
Molecule details ›
Chain: G
Length: 24 nucleotides
Theoretical weight: 7.3 KDa
RNA Chain: R
Molecule details ›
Chain: R
Length: 10 nucleotides
Theoretical weight: 3.3 KDa

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.98Å
Relevant EMDB volumes: EMD-21850

Quick links

Citations

3 review citations

Diverse molecular mechanisms of transcription regulation by the bacterial alarmone ppGpp.
Travis et al. (2022)
2 more

1 mention without citation

Structural Basis for Virulence Activation of Francisella tularensis.
Travis et al. (2021)