› 6wn4

X-ray diffraction
2.8Å resolution

Structural basis for the binding of monoclonal antibody 5D2 to the tryptophan-rich lipid-binding loop in lipoprotein lipase

Released:
DOI: 10.2210/pdb6wn4/pdb
PDB entry 6wn4 coloured by chain, front view.
PDB entry 6wn4 coloured by chain, side view.
PDB entry 6wn4 coloured by chain, top view.
Source organisms:
Primary publication:
The structural basis for monoclonal antibody 5D2 binding to the tryptophan-rich loop of lipoprotein lipase.
Luz JG, Beigneux AP, Asamoto DK, He C, Song W, Allan CM, Morales J, Tu Y, Kwok A, Cottle T, Meiyappan M, Fong LG, Kim JE, Ploug M, Young SG, Birrane G
J Lipid Res 61 1347-1359 (2020)
PMID: 32690595

Function and Biology Details

Reactions catalysed: 
Phosphatidylcholine + H(2)O = 2-acylglycerophosphocholine + a carboxylate
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209298 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
5D2 FAB HEAVY CHAIN Chains: A, H
Molecule details ›
Chains: A, H
Length: 222 amino acids
Theoretical weight: 24.3 KDa
Source organism: Mus musculus
5D2 FAB LIGHT CHAIN Chains: B, L
Molecule details ›
Chains: B, L
Length: 214 amino acids
Theoretical weight: 23.37 KDa
Source organism: Mus musculus
Lipoprotein lipase Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 amino acids
Theoretical weight: 1.77 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P06858 (Residues: 410-423; Coverage: 3%)
Gene names: LIPD, LPL

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P21
Unit cell:
a: 51.624Å b: 67.745Å c: 130.073Å
α: 90° β: 94.369° γ: 90°
R-values:
R R work R free
0.21 0.208 0.243
Expression system: Not provided

Quick links

Citations

2 review citations

GPIHBP1 and ANGPTL4 Utilize Protein Disorder to Orchestrate Order in Plasma Triglyceride Metabolism and Regulate Compartmentalization of LPL Activity.
Kristensen et al. (2021)
1 more

2 mentions without citation

Current Knowledge on Mammalian Phospholipase A1, Brief History, Structures, Biochemical and Pathophysiological Roles.
Yaginuma et al. (2022)
1 more