› 6wrv

X-ray diffraction
2.47Å resolution

Crystal structure of computationally designed protein 3DS18 in complex with the human Transferrin receptor ectodomain

Released:
DOI: 10.2210/pdb6wrv/pdb
PDB entry 6wrv coloured by chain, front view.
PDB entry 6wrv coloured by chain, side view.
PDB entry 6wrv coloured by chain, top view.
Source organisms:
Primary publication:
Transferrin receptor targeting by de novo sheet extension.
Sahtoe DD, Coscia A, Mustafaoglu N, Miller LM, Olal D, Vulovic I, Yu TY, Goreshnik I, Lin YR, Clark L, Busch F, Stewart L, Wysocki VH, Ingber DE, Abraham J, Baker D
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33879614

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136221 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Transferrin receptor protein 1, serum form Chains: A, B, E
Molecule details ›
Chains: A, B, E
Length: 639 amino acids
Theoretical weight: 71.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02786 (Residues: 121-759; Coverage: 84%)
Gene name: TFRC
Sequence domains:
Computationally designed protein 3DS18 Chains: C, D, F
Molecule details ›
Chains: C, D, F
Length: 94 amino acids
Theoretical weight: 11.03 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P42212
Unit cell:
a: 256.079Å b: 256.079Å c: 128.084Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.221 0.236
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Human organs-on-chips for disease modelling, drug development and personalized medicine.
Ingber DE. (2022)
4 more

2 mentions without citation

Transferrin receptor targeting by de novo sheet extension.
Sahtoe et al. (2021)
1 more