› 6wrx

X-ray diffraction
3.07Å resolution

Crystal structure of computationally designed protein 2DS25.1 in complex with the human Transferrin receptor ectodomain

Released:
DOI: 10.2210/pdb6wrx/pdb
PDB entry 6wrx coloured by chain, front view.
PDB entry 6wrx coloured by chain, side view.
PDB entry 6wrx coloured by chain, top view.
Source organisms:
Primary publication:
Transferrin receptor targeting by de novo sheet extension.
Sahtoe DD, Coscia A, Mustafaoglu N, Miller LM, Olal D, Vulovic I, Yu TY, Goreshnik I, Lin YR, Clark L, Busch F, Stewart L, Wysocki VH, Ingber DE, Abraham J, Baker D
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33879614

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136219 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Transferrin receptor protein 1, serum form Chains: A, B
Molecule details ›
Chains: A, B
Length: 640 amino acids
Theoretical weight: 71.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02786 (Residues: 121-760; Coverage: 84%)
Gene name: TFRC
Sequence domains:
Computationally designed protein 2DS25.1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 88 amino acids
Theoretical weight: 10.29 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN, BMA
Carbohydrate polymer
3 bound ligands:
Ligand CA 2 x CA
Ligand NAG 1 x NAG
Ligand MAN 1 x MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P3221
Unit cell:
a: 137.425Å b: 137.425Å c: 280.158Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.193 0.225
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Human organs-on-chips for disease modelling, drug development and personalized medicine.
Ingber DE. (2022)
4 more