› 6wsa

X-ray diffraction
2.05Å resolution

Crystal structure of a betaine aldehyde dehydrogenase from Burkholderia pseudomallei without cofactor

Released:
DOI: 10.2210/pdb6wsa/pdb
PDB entry 6wsa coloured by chain, front view.
PDB entry 6wsa coloured by chain, side view.
PDB entry 6wsa coloured by chain, top view.
Source organism: Burkholderia pseudomallei 1710b
Primary publication:
Crystal structure of betaine aldehyde dehydrogenase from Burkholderia pseudomallei.
Beard DK, Subramanian S, Abendroth J, Dranow DM, Edwards TE, Myler PJ, Asojo OA
Acta Crystallogr F Struct Biol Commun 78 45-51 (2022)
PMID: 35102892

Function and Biology Details

Reaction catalysed: 
Betaine aldehyde + NAD(+) + H(2)O = betaine + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174644 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Betaine aldehyde dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 496 amino acids
Theoretical weight: 53.13 KDa
Source organism: Burkholderia pseudomallei 1710b
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JLL8 (Residues: 1-489; Coverage: 100%)
Gene names: BURPS1710b_A0376, betB
Sequence domains: Aldehyde dehydrogenase family

Ligands and Environments

3 bound ligands:
Ligand SIN 1 x SIN
Ligand EDO 13 x EDO
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P6222
Unit cell:
a: 107.86Å b: 107.86Å c: 233.53Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.142 0.14 0.173
Expression system: Escherichia coli

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Citations

1 mention without citation

Crystal structure of betaine aldehyde dehydrogenase from Burkholderia pseudomallei.
Beard et al. (2022)