› 6wsk

X-ray diffraction
1.55Å resolution

Crystal Structure of the Cannabinoid Receptor 1 Interacting Protein 1a (CRIP1a)

Released:
DOI: 10.2210/pdb6wsk/pdb
PDB entry 6wsk coloured by chain, front view.
PDB entry 6wsk coloured by chain, side view.
PDB entry 6wsk coloured by chain, top view.
Source organisms:
Primary publication:
Cannabinoid receptor interacting protein 1a interacts with myristoylated Gαi N terminus via a unique gapped β-barrel structure.
Booth WT, Clodfelter JE, Leone-Kabler S, Hughes EK, Eldeeb K, Howlett AC, Lowther WT
J Biol Chem 297 101099 (2021)
PMID: 34418434

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133011 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CB1 cannabinoid receptor-interacting protein 1; Endolysin Chain: A
Molecule details ›
Chain: A
Length: 346 amino acids
Theoretical weight: 39.21 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 1-161; Coverage: 98%)
  • Canonical: Q5M7A7 (Residues: 1-164; Coverage: 100%)
Gene names: Cnrip1, E
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P41212
Unit cell:
a: 68.177Å b: 68.177Å c: 146.648Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.224
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Endocannabinoid signaling in the central nervous system.
Martinez Ramirez et al. (2023)

2 mentions without citation

Effects of Environmental Heat Load on Endocannabinoid System Components in Adipose Tissue of High Yielding Dairy Cows.
Kra et al. (2022)
1 more