› 6wtf

X-ray diffraction
2.19Å resolution

Structure of radical S-adenosylmethionine methyltransferase, TsrM, from Kitasatospora setae with tryptophan substrate and SAM analog (aza-SAM) bound

Released:
DOI: 10.2210/pdb6wtf/pdb
PDB entry 6wtf coloured by chain, front view.
PDB entry 6wtf coloured by chain, side view.
PDB entry 6wtf coloured by chain, top view.
Source organism: Kitasatospora setae KM-6054
Primary publication:
Structural basis for non-radical catalysis by TsrM, a radical SAM methylase.
Knox HL, Chen PY, Blaszczyk AJ, Mukherjee A, Grove TL, Schwalm EL, Wang B, Drennan CL, Booker SJ
Nat Chem Biol 17 485-491 (2021)
PMID: 33462497

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122936 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan-C2-methyltransferase containing B12-binding domain Chains: A, B
Molecule details ›
Chains: A, B
Length: 575 amino acids
Theoretical weight: 64.14 KDa
Source organism: Kitasatospora setae KM-6054
Expression system: Escherichia coli
UniProt:
  • Canonical: E4N8S5 (Residues: 1-575; Coverage: 100%)
Gene name: KSE_17820
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand SF4 2 x SF4
Ligand B12 2 x B12
Ligand SA8 2 x SA8
Ligand TRP 2 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 50.355Å b: 103.085Å c: 105.757Å
α: 90° β: 94.77° γ: 90°
R-values:
R R work R free
0.213 0.211 0.249
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Cobalamin-Dependent Radical S-Adenosylmethionine Enzymes: Capitalizing on Old Motifs for New Functions.
Bridwell-Rabb et al. (2022)
3 more

6 mentions without citation

Computational Approaches: An Underutilized Tool in the Quest to Elucidate Radical SAM Dynamics.
Blue et al. (2021)
5 more