› 6wu9

Electron Microscopy
2.9Å resolution

50S subunit of 70S Ribosome Enterococcus faecalis MultiBody refinement

Released:
DOI: 10.2210/pdb6wu9/pdb
PDB entry 6wu9 coloured by chain, front view.
PDB entry 6wu9 coloured by chain, side view.
PDB entry 6wu9 coloured by chain, top view.
Source organism: Enterococcus faecalis OG1RF
Primary publication:
Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis.
Murphy EL, Singh KV, Avila B, Kleffmann T, Gregory ST, Murray BE, Krause KL, Khayat R, Jogl G
OpenAccess logo Sci Rep 10 16301 (2020)
PMID: 33004869
Related structures: EMD-21907

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 27-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-300358 (preferred)
Entry contents:
25 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (27 distinct):
Large ribosomal subunit protein uL3 Chain: D
Molecule details ›
Chain: D
Length: 207 amino acids
Theoretical weight: 22.5 KDa
Source organism: Enterococcus faecalis OG1RF
UniProt:
  • Canonical: Q839G4 (Residues: 2-208; Coverage: 99%)
Gene names: EF_0206, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: E
Molecule details ›
Chain: E
Length: 206 amino acids
Theoretical weight: 22.38 KDa
Source organism: Enterococcus faecalis OG1RF
UniProt:
  • Canonical: Q839G3 (Residues: 2-207; Coverage: 100%)
Gene names: EF_0207, rplD
Sequence domains: Ribosomal protein L4/L1 family
Large ribosomal subunit protein uL5 Chain: F
Large ribosomal subunit protein uL6 Chain: G
Large ribosomal subunit protein uL13 Chain: K
Large ribosomal subunit protein uL14 Chain: L
Large ribosomal subunit protein uL15 Chain: M
Large ribosomal subunit protein uL16 Chain: N
Large ribosomal subunit protein bL17 Chain: O
Large ribosomal subunit protein uL18 Chain: P
Large ribosomal subunit protein bL19 Chain: Q
Large ribosomal subunit protein bL20 Chain: R
Large ribosomal subunit protein bL21 Chain: S
Large ribosomal subunit protein uL22 Chain: T
Large ribosomal subunit protein uL23 Chain: U
Large ribosomal subunit protein uL24 Chain: V
Large ribosomal subunit protein bL27 Chain: X
Large ribosomal subunit protein bL28 Chain: Y
Large ribosomal subunit protein uL29 Chain: Z
Large ribosomal subunit protein uL30 Chain: 0
Large ribosomal subunit protein bL32C Chain: 2
Large ribosomal subunit protein bL33C Chain: 3
Large ribosomal subunit protein bL34 Chain: 4
Large ribosomal subunit protein bL35 Chain: 5
Large ribosomal subunit protein bL36 Chain: 6
23S ribosomal RNA Chain: A
5S ribosomal RNA Chain: B

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.9Å
Relevant EMDB volumes: EMD-21907

Quick links

Citations

1 review citation

Mechanisms of catalytic RNA molecules.
Alonso et al. (2021)
8 other articles