› 6wur

X-ray diffraction
2.88Å resolution

Crystal structure of PRL-2 phosphatase C101D mutant in complex with the Bateman domain of CNNM3 magnesium transporter

Released:
DOI: 10.2210/pdb6wur/pdb
PDB entry 6wur coloured by chain, front view.
PDB entry 6wur coloured by chain, side view.
PDB entry 6wur coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of PRL phosphatase C104D mutant in complex with the Bateman domain of CNNM magnesium transporter
Kozlov G, Funato Y, Chen S, Zhang Z, Illes K, Miki H, Gehring K
J. Biol. Chem. (2020)

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171465 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein tyrosine phosphatase type IVA 2 Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12974 (Residues: 1-167; Coverage: 100%)
Gene names: BM-008, PRL2, PTP4A2, PTPCAAX2
Sequence domains: Protein-tyrosine phosphatase
Metal transporter CNNM3 Chain: B
Molecule details ›
Chain: B
Length: 155 amino acids
Theoretical weight: 17.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NE01 (Residues: 309-452; Coverage: 20%)
Gene names: ACDP3, CNNM3
Sequence domains: CBS domain

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: I222
Unit cell:
a: 56.857Å b: 124.544Å c: 153.413Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.238 0.295
Expression system: Escherichia coli

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