› 6wxl

Electron Microscopy
2.76Å resolution

Cryo-EM structure of the VRC315 clinical trial, vaccine-elicited, human antibody 1D12 in complex with an H7 SH13 HA trimer

Released:
DOI: 10.2210/pdb6wxl/pdb
PDB entry 6wxl coloured by chain, front view.
PDB entry 6wxl coloured by chain, side view.
PDB entry 6wxl coloured by chain, top view.
Source organisms:
Primary publication:
Structure of an influenza group 2-neutralizing antibody targeting the hemagglutinin stem supersite.
Cheung CS, Gorman J, Andrews SF, Rawi R, Reveiz M, Shen CH, Wang Y, Harris DR, Nazzari AF, Olia AS, Raab J, Teng IT, Verardi R, Wang S, Yang Y, Chuang GY, McDermott AB, Zhou T, Kwong PD
Structure 30 993-1003.e6 (2022)
PMID: 35489332
Related structures: EMD-21961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207217 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemagglutinin Chains: A, C, G
Molecule details ›
Chains: A, C, G
Length: 321 amino acids
Theoretical weight: 35.04 KDa
Source organism: Influenza A virus (A/Shanghai/JS01/2013(H7N9))
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A067Y6L0 (Residues: 19-339; Coverage: 57%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin Chains: B, D, I
Molecule details ›
Chains: B, D, I
Length: 221 amino acids
Theoretical weight: 25.23 KDa
Source organism: Influenza A virus (A/Shanghai/JS01/2013(H7N9))
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A067Y6L0 (Residues: 340-560; Coverage: 40%)
Gene name: HA
Sequence domains: Haemagglutinin
1D12 Light chain Chains: E, J, L
Molecule details ›
Chains: E, J, L
Length: 221 amino acids
Theoretical weight: 23.97 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
1D21 Heavy chain Chains: F, H, K
Molecule details ›
Chains: F, H, K
Length: 232 amino acids
Theoretical weight: 25.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand NAG 9 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.76Å
Relevant EMDB volumes: EMD-21961
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Co-immunization with hemagglutinin stem immunogens elicits cross-group neutralizing antibodies and broad protection against influenza A viruses.
Moin et al. (2022)

2 mentions without citation

Structure of an influenza group 2-neutralizing antibody targeting the hemagglutinin stem supersite.
Cheung et al. (2022)
1 more