› 6x1q

Electron Microscopy
1.8Å resolution

1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope

Released:
DOI: 10.2210/pdb6x1q/pdb
PDB entry 6x1q coloured by chain, front view.
PDB entry 6x1q coloured by chain, side view.
PDB entry 6x1q coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
1.8 Å resolution structure of β-galactosidase with a 200 kV CRYO ARM electron microscope.
Merk A, Fukumura T, Zhu X, Darling JE, Grisshammer R, Ognjenovic J, Subramaniam S
IUCrJ 7 639-643 (2020)
PMID: 32695410
Related structures: EMD-21995

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1021 amino acids
Theoretical weight: 116.18 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P00722 (Residues: 3-1023; Coverage: 100%)
Gene names: JW0335, b0344, lacZ
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MG 8 x MG
Ligand NA 8 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 1.8Å
Relevant EMDB volumes: EMD-21995

Quick links

Citations

2 review citations

Direct Visualization of Actin Filaments and Actin-Binding Proteins in Neuronal Cells.
Jung et al. (2020)
1 more

3 mentions without citation

1.8 Å resolution structure of β-galactosidase with a 200 kV CRYO ARM electron microscope.
Merk et al. (2020)
2 more